Hasipuffer im Forum sagte was ueber einen HBA Test… Da hab ich mal gegoogelt und bin auf folgendes gestossen: SPA und HBA Test. SPA waere der sogenannte Hamster-Ei Test.

Zitat:

Sperm Penetration Assay (SPA)

The SPA is a multistep laboratory test that offers a biological assessment of human sperm fertilizing ability. Specifically, 4 distinct processes must occur for sperm-oocyte fertilization: capacitation, acrosome reaction, penetration of the ooplasm, and chromatin decondensation within the ooplasm. The SPA uses a zona-free hamster egg as an in vitro model for sperm-oocyte interaction. (The zona pellucida is a thick, glassy membrane surrounding the oocyte that maintains species specificity for fertilization. Removal of the zona pellucida from hamster ova permits their use as a model for sperm oocyte interaction.) The test is performed by incubating a number of zona-free hamster eggs with human sperm for several hours. According to the percentage of ova penetrated, the semen sample is rated as being in a potentially “fertile” or “infertile” range. Aspects of sperm function not assessed by the SPA include the capacity of sperm to penetrate the cervical barrier or their ability to bind to the zona pellucida of human ova.

The sperm penetration assay, alternatively referred to as the hamster oocyte penetration test or the zona-free hamster egg test, was initially developed in 1976. Although it has been used as a test for male infertility, the test has been controversial due to variability in procedures used by different laboratories, poor reproducibility of test outcomes, and uncertainty regarding the range of normal values.

Hyaluronan Binding Assay (HBA)

The HBA is a qualitative assay for the maturity of sperm in a fresh semen sample. The assay is based on the ability of mature, but not immature, sperm to bind hyaluronan, the main mucopolysaccharide of the cumulus oophorus matrix and a component of human follicular fluid. Research has shown that hyaluronan-binding capacity is acquired late in the sperm maturation process; immature sperm lack this ability. Therefore, a low level of sperm binding to hyaluronan suggests that there is a low proportion of mature sperm in the sample. Similar to the sperm penetration assay, it has been suggested that the HBA assay may be used to determine the need for an intracytoplasmic sperm injection procedure (ICSI) as part of an assisted reproductive technique. The HBA is a laboratory test that has received U.S. Food and Drug Administration (FDA) clearance through the 510(k) approval process. The FDA-labeled indications are as follows:

1. As a component of the standard analysis of semen in the diagnosis of suspected male infertility.

2. As a component of analyses for determining the proper course of in vitro fertilization treatment of infertility.

In 2006, a product containing hyaluronan (SpermSlow™) received 510(k) marketing clearance for the separation of mature from immature sperm prior to intracytoplasmic sperm injection (ICSI).

Denke, dass die KiWu den HBA Test meint. Werde da jetzt weiter googeln und aufschreiben, was ich finde…

http://www.midatlanticdiagnostics.com/products/micro/picsi.htm

http://www.midatlanticdiagnostics.com/products/sperm/hba.htm

http://www.klein-putz.net/forum/viewtopic.php?t=46479

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